su dhl4  (DSMZ)

Journal: Hereditas

Article Title: Damnacanthus giganteus extract block diffuse large b-cell lymphoma proliferation and EMT by regulating mitochondrial dysfunction and glycolysis

doi: 10.1186/s41065-025-00531-3

Figure Lengend Snippet: DGE inhibits cell proliferation and promotes DOX sensitivity in DLBCL cells. A : CCK-8 assay to detect the effect of different concentrations of DGE on the viability of GM12878 cells; B : CCK-8 assay to detect the effect of different concentrations of DGE on the viability of SU-DHL4 cells; C : EdU assay to detect the effect of different concentrations of DGE on the proliferation rate of the cells; D : Colony formation assay to detect the effect of different concentrations of DGE on the ability of the cells to form clones; E : AnnexinV-PI double staining assay to detect the effect of different concentrations of DGE on the apoptosis rate of cells; F : CCK-8 assay to detect the effect of different concentrations of DGE on the sensitivity of SU-DHL4 cells to DOX. Cells were treated with DGE or DOX for 24 h, DOX (0.5 µM, 24 h) was used as a positive control. Each group of experiments was repeated independently 3 times, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Normal human B lymphocytes (GM12878) with human DLBCL cell line SU-DHL4 (ATCC, VA, USA) were placed in Iscove’s modifed Dulbecco’s medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco) and penicillin/streptomycin 100 U/mL and cultured at 37 °C, 5% CO 2 .

Techniques: CCK-8 Assay, EdU Assay, Colony Assay, Clone Assay, Double Staining, Positive Control

Journal: Hereditas

Article Title: Damnacanthus giganteus extract block diffuse large b-cell lymphoma proliferation and EMT by regulating mitochondrial dysfunction and glycolysis

doi: 10.1186/s41065-025-00531-3

Figure Lengend Snippet: DGE impairs metastasis and EMT in DLBCL cells. A - B : Transwell assay to detect the effect of different concentrations of DGE on cell migration and invasion ability; C : Western blot to detect the effect of different concentrations of DGE on the expression levels of cellular E-cadherin, N-cadherin and Vimentin. Cells were treated with DGE or DOX for 24 h, DOX (0.5 µM, 24 h) was used as a positive control. Each group of experiments was repeated independently 3 times, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Normal human B lymphocytes (GM12878) with human DLBCL cell line SU-DHL4 (ATCC, VA, USA) were placed in Iscove’s modifed Dulbecco’s medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco) and penicillin/streptomycin 100 U/mL and cultured at 37 °C, 5% CO 2 .

Techniques: Transwell Assay, Migration, Western Blot, Expressing, Positive Control

Journal: Hereditas

Article Title: Damnacanthus giganteus extract block diffuse large b-cell lymphoma proliferation and EMT by regulating mitochondrial dysfunction and glycolysis

doi: 10.1186/s41065-025-00531-3

Figure Lengend Snippet: DGE and DOX synergistically inhibit DLBCL cell growth in a mouse xenograft model. A : Schematic diagram of the in vivo experiment. Mice bearing tumors were treated with vehicle control, DGE (100 mg/kg/once per day, i.g.), DOX (1.5 mg/kg/once per week, i.p. ), or DGE + DOX for 21 days. n = 3 mice per group. B : Representative pictures of transplanted tumors in nude mice; C - D : The volume and weight of transplanted tumors in nude mice; E - F : Immunohistochemical assessment of the positive expression levels of Ki67 and N-cadherin proteins in the tissues of transplanted tumors in nude mice. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Normal human B lymphocytes (GM12878) with human DLBCL cell line SU-DHL4 (ATCC, VA, USA) were placed in Iscove’s modifed Dulbecco’s medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco) and penicillin/streptomycin 100 U/mL and cultured at 37 °C, 5% CO 2 .

Techniques: In Vivo, Control, Immunohistochemical staining, Expressing

Journal: Hereditas

Article Title: Damnacanthus giganteus extract block diffuse large b-cell lymphoma proliferation and EMT by regulating mitochondrial dysfunction and glycolysis

doi: 10.1186/s41065-025-00531-3

Figure Lengend Snippet: DGE regulates mitochondrial function and glycolysis in DLBCL cells. A : MitoSOX Red staining to assess the effect of different concentrations of DGE on mitochondrial ROS; B : Flow cytometry analysis of the effect of different concentrations of DGE on MMP; C : Kits to detect the effects of different concentrations of DGE on cellular ATP production; D : Western blot to detect the effect of different concentrations of DGE on the expression levels of cellular PINK1 and PRKN; E - F : Kits to detect the effects of different concentrations of DGE on cellular glucose uptake and lactate production. Cells were treated with DGE for 24 h. Each group of experiments was repeated independently 3 times, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Normal human B lymphocytes (GM12878) with human DLBCL cell line SU-DHL4 (ATCC, VA, USA) were placed in Iscove’s modifed Dulbecco’s medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco) and penicillin/streptomycin 100 U/mL and cultured at 37 °C, 5% CO 2 .

Techniques: Staining, Flow Cytometry, Western Blot, Expressing